HIV HbsAg HCV combo quick test cassette
HIV HbsAg HCV Combo Rapid Test Cassette (Ropa Rakazara/Serum/Plasma)
SUMMARY
Nzira huru yekuona utachiona neHCV/HIV/HbsAg ndeyekucherechedza kuvapo kwemasoja ekudzivirira chirwere kuutachiona nenzira yeEIA inoteverwa nekusimbiswa neWestern Blot.Iyo One Danho Bvunzo iri nyore, rinoona hunhu bvunzo unoona masoja ekudzivirira chirwere mumunhu Wese Ropa/ serum/plasma.Muedzo unobva pa immunochromatography uye unogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.
ZVIRI KUSHANDISA
Iyo One Step Test iColloidal Goridhe yakakwidziridzwa, nekukurumidza Immunochromatoraphic Assay yemhando yekuonekwa kwemasoja ekudzivirira chirwere kuHCV/HIV/HBsAG muMunhu Wese Ropa / Serum / Plasma.Muedzo uyu bvunzo yekuongorora uye zvese zvakanaka zvinofanirwa kusimbiswa uchishandisa imwe bvunzo yakaita seWestern Blot.Muedzo wakaitirwa Healthcare Professional kushandiswa chete.Zvese zviri zviviri bvunzo nemhedzisiro yekuyedzwa zvinotarisirwa kuti zvishandiswe nevekurapa nenyanzvi dzemutemo chete, kunze kwekunge zvabvumidzwa neimwe nzira nemutemo munyika inoshandiswa.Muedzo haufanirwe kushandiswa pasina kutarisirwa kwakakodzera.
REAGENTS NEZVINHU ZVINOPIWA
Yedza mudziyo wega foil wakaiswa muhomwe ine desiccant
• Plastic dropper.
• Sample Diluent
• package Isa
ZVINHU ZVINODIWA ASI ZVISINA KUPIWA
Positive and negative controls (inowanikwa sechinhu chakasiyana)
KUCHENGA NEKUTSIGAMA
Matest kits anofanirwa kuchengetwa pa2-30 ℃ muhomwe yakavharwa uye pasi pemamiriro akaoma.
YAMBIRO UYE CHENJERERO
1) Zvose zvakanaka zvinoguma zvinofanira kusimbiswa neimwe nzira.
2) Bata zvienzaniso zvese sekunge zvinogona kutapukira.Pfeka magirovhosi uye zvipfeko zvekudzivirira paunenge uchibata sampuli.
3) Midziyo inoshandiswa pakuyedzwa inofanirwa kuve yakavharwa isati yaraswa.
4) Usashandise kit zvinhu kupfuura mazuva avo ekupera.
5) Usachinjane mareagents kubva kune akasiyana mijenya.
SAMPLE KUCHENGA UYE KUCHENGA
1) Unganidza Ropa Rakazara / Serum / Plasma zvienzaniso zvinotevera maitiro enguva dzose ekiriniki murabhoritari.
2) Kuchengeta: Ropa Rakazara harigone kuomeswa nechando.Muenzaniso unofanirwa kuiswa mufiriji kana usina kushandiswa zuva rimwechete rekuunganidza.Mienzaniso inofanira kuiswa muchando kana isina kushandiswa mukati memazuva matatu ekuunganidza.Dzivisa kuomesa nechando uye kunyunguduka zvienzaniso kanopfuura ka2-3 usati washandisa.0.1% yeSodium Azide inogona kuwedzerwa kumuenzaniso sekuchengetedza pasina kukanganisa mhedzisiro yeassay.
ASAY PROCEDURE
1) Uchishandisa iyo yakavharirwa yepurasitiki inodonhedza sampuli, buritsa 1 donho (10μl) reRopa Rakazara / Serum / Plasma kune yakatenderedza sampuli tsime rebvunzo kadhi.
2) Wedzera madonhwe maviri eSample Diluent kune sampuli tsime, pakarepo mushure meiyo specimen yawedzerwa, kubva kune inodonha tip diluent vial (kana zvese zvirimo kubva kune imwechete bvunzo ampule).
3) Dudzira mhinduro dzebvunzo pamaminitsi gumi nemashanu.
Notes:
1) Kuisa huwandu hwakakwana hwesample diluent kwakakosha kune inoshanda bvunzo mhinduro.Kana kutama (kunyorova kwemembrane) kusingaonekwe muhwindo rekuyedza mushure meminiti imwe, wedzera rimwe donhwe re diluent kune tsime remuenzaniso.
2) Mhedzisiro yakanaka inogona kuoneka nekukurumidza seminiti imwe yesampuli ine huwandu hwakanyanya hwemasoja ekudzivirira eHCV.
3) Usadudzire zvabuda mushure memaminitsi makumi maviri
KUVERENGA ZVAKABUDZWA ZVIYEDZO
1)Positive: Zvese zviri zviviri purplish red test bhendi uye purplish red control bhendi zvinoonekwa pane membrane.Kudzikira kwemasoja ekudzivirira chirwere, ndiko kuderera kwebhendi rekuyedza.
2) Negative: Iyo chete purplish red control bhendi inoonekwa pane membrane.Kusavapo kwebhendi rekuedza kunoratidza mhedzisiro yakaipa.
3)Mhedzisiro isiriyo:Panofanira kugara paine purplish red control bhendi mudunhu rekutonga, zvisinei nemhedzisiro yebvunzo.Kana bhendi rekudzora risingaonekwe, bvunzo inoonekwa seyakashata.Dzokorora bvunzo uchishandisa mudziyo mutsva wekuyedza.
Ongorora: Zvakajairika kuva nebhendi rakarerutswa zvishoma rine masampuli akasimba akasimba, chero achinyatso kuoneka.
LIMITATION
1) Chete yakajeka, nyowani, yemahara inoyerera Yese Ropa / Serum / Plasma inogona kushandiswa muyedzo iyi.
2) Fresh samples ndiye akanakisa asi akaomeswa nechando anogona kushandiswa.Kana sampuli yakaomeswa nechando, inofanira kubvumidzwa kunyunguduka panzvimbo yakatwasuka uye ichitariswa kuti haina mvura here.Ropa rose harigoni kuomeswa nechando.
3) Usadenha sampuli.Isa pipette pazasi pepamusoro peiyo sampuli kuti utore Specimen.
