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chigadzirwa

ONE STEP HCV TEST (Ropa Rakazara/Serum/Plasma)

Tsanangudzo Pfupi


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ONE STEP HCV TEST (Ropa Rakazara/Serum/Plasma)

hcv rna
anti hcv test
hcv antibody
hcv test
hepatitis c test

SUMMARY

Nzira huru yekuona utachiona neHCV ndeyekucherechedza kuvepo kwemasoja ekudzivirira chirwere ichi nenzira yeEIA inoteverwa nekusimbiswa neWestern Blot. Iyo One Danho HCV Bvunzo iri nyore, inoonekwa yemhando bvunzo inoona masoja ekudzivirira chirwere mumunhu Wese Ropa/ serum/plasma. Muedzo unobva pa immunochromatography uye unogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.

ZVIRI KUSHANDISA

Iyo Imwe Nhanho HCV Muedzo ndeyeColloidal Goridhe yakakwidziridzwa, nekukurumidza Immunochromatoraphic Assay yemhando yekuona masoja ekudzivirira chirwere cheHepatitis C Virus (HCV) muMunhu Wese Ropa / Serum / Plasma. Muedzo uyu bvunzo yekuongorora uye zvese zvakanaka zvinofanirwa kusimbiswa uchishandisa imwe bvunzo yakaita seWestern Blot. Muedzo wakaitirwa Healthcare Professional kushandiswa chete. Zvese zviri zviviri kuyedzwa uye zvabuda mukuyedzwa zvakaitirwa kuti zvishandiswe nevekurapa nenyanzvi dzemutemo chete, kunze kwekunge zvabvumidzwa nemutemo munyika inoshandiswa. Muedzo haufanirwe kushandiswa pasina kutarisirwa kwakakodzera.

ZVINHU ZVINOITA KUTI

Chiyero chinotanga nesample yakashandiswa kune sample tsime uye nekuwedzera kweiyo yakapihwa sampuli diluent nekukasira. HCV antigen-Colloidal Goridhe conjugate yakaiswa mumuenzaniso pad inopindirana neHCV antibody iripo muserum kana plasma, ichigadzira conjugate/HCV antibody complex. Sezvo musanganiswa uchitenderwa kutama uchitevedza bvunzo tambo, conjugate/HCV antibody complex inotorwa neantibody-binding protein A isingafambire pamembrane ichiita bhendi rine ruvara mudunhu rebvunzo. Sample yakashata haiburitse mutsara wekuyedza nekuda kwekushaikwa kweColloidal Gold conjugate/HCV antibody complex. Maantigen anoshandiswa muyedzo mapuroteni anosanganisirwa anoenderana neakanyanya immunoreactive matunhu eHCV. Bhandi rekutonga rine mavara munharaunda yekutonga rinoonekwa pakupera kwemaitiro ekuedza zvisinei nemugumisiro wekuedzwa. Iri bhendi rekutonga mhedzisiro yeColloidal Goridhe conjugate inosunga kune anti-HCV antibody isingafambiswe pa membrane. Mutsetse wekutonga unoratidza kuti Colloidal Gold conjugate inoshanda. Kusavapo kwebhendi rekudzora kunoratidza kuti bvunzo haina kunaka.

REAGENTS NEZVINHU ZVINOPIWA

Yedza mudziyo wega foil wakaiswa muhomwe ine desiccant

• Plastic dropper.

• Sample Diluent

• package Isa

ZVINHU ZVINODIWA ASI ZVISINA KUPIWA

Positive and negative controls (inowanikwa sechinhu chakasiyana)

KUCHENGA NEKUTSIGAMA

Matest kits anofanirwa kuchengetwa pa2-30 ℃ muhomwe yakavharwa uye pasi pemamiriro akaoma.

YAMBIRO UYE ZVOKUDZIVIRIRA

1) Zvese zvakanaka mhedzisiro dzinofanirwa kusimbiswa neimwe nzira.

2) Bata zvienzaniso zvese sekunge zvinogona kutapukira. Pfeka magirovhosi uye zvipfeko zvekudzivirira paunenge uchibata sampuli.

3) Midziyo inoshandiswa pakuyedzwa inofanirwa kuve yakavharwa isati yaraswa.

4) Usashandise kit zvinhu kupfuura mazuva avo ekupera.

5) Usachinjane mareagents kubva kune akasiyana mijenya.

SAMPLE KUCHENGA UYE KUCHENGA

1) Unganidza Ropa Rakazara / Serum / Plasma zvienzaniso zvinotevera maitiro enguva dzose ekiriniki murabhoritari.

2) Kuchengeta: Ropa rose harigone kuomeswa nechando. Muenzaniso unofanirwa kuiswa mufiriji kana usina kushandiswa zuva rimwechete rekuunganidza. Mienzaniso inofanira kuiswa muchando kana isina kushandiswa mukati memazuva matatu ekuunganidza. Dzivisa kuomesa nechando uye kunyunguduka zvienzaniso kanopfuura ka2-3 usati washandisa. 0.1% yeSodium Azide inogona kuwedzerwa kumuenzaniso sekuchengetedza pasina kukanganisa mhedzisiro yeassay.

ASAY PROCEDURE

1) Uchishandisa iyo yakavharirwa yepurasitiki inodonhedza sampuli, buritsa 1 donho (10μl) reRopa Rakazara / Serum / Plasma kune yakatenderedza sampuli tsime rebvunzo kadhi.

2) Wedzera madonhwe maviri eSample Diluent kune sampuli tsime, pakarepo mushure meiyo specimen yawedzerwa, kubva kune inodonha tip diluent vial (kana zvese zvirimo kubva kune imwechete bvunzo ampule).

3) Dudzira mhinduro dzebvunzo pamaminitsi gumi nemashanu. 

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Notes:

1) Kuisa huwandu hwakakwana hwesample diluent kwakakosha kune inoshanda bvunzo mhinduro. Kana kutama (kunyorova kwemembrane) kusingaonekwe muhwindo rekuyedza mushure meminiti imwe, wedzera rimwe donhwe re diluent kune sampuli tsime.

2) Mhedzisiro yakanaka inogona kuoneka nekukurumidza seminiti imwe yesampuli ine huwandu hwakanyanya hwemasoja ekudzivirira eHCV.

3) Usadudzire zvabuda mushure memaminitsi makumi maviri

KUVERENGA ZVINOBVA ZVAKAITWA

1) Positive: Zvese zviri zviviri purplish red test bhendi uye purplish red control bhendi zvinoonekwa pane membrane. Kudzikira kwemasoja emasoja, ndiko kuderera kwebhendi rekuyedza.

2) Negative: Iyo chete purplish red control bhendi inoonekwa pane membrane. Kusavapo kwebhendi rekuedza kunoratidza mhedzisiro yakaipa.

3) Mhedzisiro isiriyo: Panofanira kugara paine purplish red control bhendi mudunhu rekutonga, zvisinei nemhedzisiro yebvunzo. Kana bhendi rekudzora risingaonekwe, bvunzo inoonekwa seyakashata. Dzokorora bvunzo uchishandisa mudziyo mutsva wekuyedza.

Ongorora: Zvakajairika kuva nebhendi rakarerutswa zvishoma rine masampuli akasimba akasimba, chero richinyatso kuoneka.

LIMITATION

1) Chete yakajeka, nyowani, yemahara inoyerera Yese Ropa / Serum / Plasma inogona kushandiswa muyedzo iyi.

2) Fresh samples ndiye akanakisa asi akaomeswa nechando anogona kushandiswa. Kana sampuli yakaomeswa nechando, inofanira kubvumidzwa kunyunguduka panzvimbo yakatwasuka uye ichitariswa kuti haina mvura here. Ropa rose harigoni kuomeswa nechando.

3) Usadenha sampuli. Isa pipette pazasi pepamusoro peiyo sampuli kuti utore iyo Specimen. 


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