Vatsvagiri veDutch vanosanganisa CRISPR uye bioluminescence muyedzo yekuyedzazvirwere zvinotapukira

Protein ichangobva kugadzirwa yehusiku inogona kukurumidzira uye kurerutsa kuongororwa kwezvirwere zvehutachiona, sekureva kwevaongorori muNetherlands.
Dzidzo yavo, yakabudiswa neChitatu mu ACS Publications, inotsanangura nzira yakaoma, imwe-nhanho yekukurumidza kuongorora viral nucleic acids uye chitarisiko chavo vachishandisa kupenya kwebhuruu kana mapuroteni egirini.
Kuzivikanwa kwehutachiona nekuona mafingerprints avo nucleic acid idanho rakakosha mukuongororwa kwekiriniki, biomedical research, uye chikafu uye kuchengetedza kuchengetedzwa kwezvakatipoteredza.Iyo yakawanda inoshandiswa yakawanda polymerase chain reaction (PCR) bvunzo ine hunyanzvi, asi inoda yakaomesesa kugadzirira kana kududzirwa kwemhedzisiro, zvichiita kuti isashande kune mamwe marongero ehutano kana zviwanikwa-zvishoma marongero.
Iri boka rinobva kuNetherlands ndiro mugumisiro wekubatana pakati pemasayendisiti kubva kumayunivhesiti nezvipatara kuti vagadzire nzira inokurumidza, inotakurika uye iri nyore kushandisa nucleic acid yekuongorora iyo inogona kushandiswa munzvimbo dzakasiyana-siyana.
Dzakafemerwa nekupenya kwezvinhinhimira, kupenya kwezvinopenya, uye nyeredzi diki dzemumvura phytoplankton, zvese zvine simba nechiitiko chinonzi bioluminescence.Uku kupenya-mu-kwa-rima kunoita kunokonzerwa nekemikari yekuita inosanganisira luciferase protein.Masayendisiti akabatanidza mapuroteni eluciferase mumasensa anoburitsa mwenje kuti afambise kutarisa kana vawana chinangwa.Nepo izvi zvichiita kuti ma sensors aya ave akanaka pakuonekwa-kwe-kutarisirwa, iye zvino haana kunzwisiswa kwakanyanya kunodiwa kuongororwa kwekiriniki.Nepo iyo CRISPR gene editing nzira inogona kupa iyi kugona, inoda matanho mazhinji uye yekuwedzera hunyanzvi michina yekuona iyo isina simba chiratidzo chinogona kuve chiripo mune yakaoma, ine ruzha samples.
Vatsvakurudzi vakawana nzira yekubatanidza puroteni yakabatana neCRISPR nechiratidzo chebioluminescent chinogona kuonekwa nekamera yedhijitari iri nyore.Kuve nechokwadi chekuti pakanga paine yakakwana RNA kana DNA sampuli yekuongorora, vaongorori vakaita recombinase polymerase amplification (RPA), nzira iri nyore inoshanda nekudziya kwekusingaperi kunosvika zana.Vakagadzira chikuva chitsva chinodaidzwa kuti Luminescent Nucleic Acid Sensor (LUNAS), umo mapuroteni maviri eCRISPR/Cas9 akanangana nezvikamu zvakasiyana-siyana zvevhairasi genome, imwe neimwe iine yakasarudzika luciferase fragment yakanamatira paari pamusoro.
Apo iyo chaiyo yehutachiona genome iyo vatsvakurudzi vari kuongorora iripo, mapuroteni maviri eCRISPR / Cas9 anosunga kune chinangwa che nucleic acid sequence;vanova vari pedyo, vachibvumira intact luciferase protein kuti iumbe uye ibudise chiedza chebhuruu pamberi pekemikari substrate..Kuzvidavirira kune substrate inopedzwa mukuita uku, vaongorori vakashandisa maitiro ekudzora akabudisa chiedza chegirini.Ichubhu inoshandura ruvara kubva kugirinhi kuenda kublue inoratidza mhedzisiro yakanaka.
Vatsvagiri vakaedza chikuva chavo nekugadzira iyo RPA-LUNAS assay, iyo inoonaSARS-CoV-2 RNApasina inonetesa RNA yekuzviparadzanisa nevamwe, uye yakaratidza mashandiro ayo ekuongorora pane nasopharyngeal swab samples kubvaCOVID 19varwere.RPA-LUNAS yakabudirira kuona SARS-CoV-2 mukati memaminitsi makumi maviri mumasamples ane RNA viral load yakaderera se200 makopi/μL.
Vatsvagiri vanotenda kuti muyedzo wavo unogona kuona zviri nyore uye zvinobudirira mamwe mavhairasi akawanda."RPA-LUNAS inoyevedza kuongororwa kwechirwere chinotapukira," vakanyora kudaro.