Chinangwa Kushandiswa

Dengue NS1 Ag-IgG/IgM Combo Test inokurumidza chromatographic immunoassay yemhando yekuona masoja ekudzivirira chirwere (IgG neIgM) uye hutachiona hwedengue NS1 antigen kuhutachiona hwedengue muSerum kana Plasma kubatsira mukuongororwa kwehutachiona hweDengue.

[SUMMARY]

Dengue fever chirwere chechirwere chinotapukira chinokonzerwa nehutachiona hwedengue hunoparadzirwa nehumhutu.Utachiona hwehutachiona hweDengue hunogona kutungamirira kuhutachiona hunowedzera, dengue fever, dengue hemorrhagic fever, dengue hemorrhagic fever.Makiriniki ekuratidzwa kwefivha yedengue anosanganisira kuerekana kwatanga, kupisa kwakanyanya, kutemwa nemusoro, tsandanyama zvakanyanya, kurwadziwa kwebhonzo nemajoini, kuputika kweganda, kubuda ropa, kuwedzera lymph node, kuderera kwemasero machena eropa, thrombocytopenia uye zvichingodaro mune vamwe varwere.Chirwere ichi chinonyanya kufarirwa munzvimbo dzinopisa uye dzinopisa, nekuti chirwere ichi chinoparadzirwa neAides mosquito, chikonzero chakakurumbira kune imwe nguva mwaka, kuve mugore rega rega kazhinji muna Chivabvu-Mbudzi, peak iri muna Chikunguru ~ Gunyana.Munzvimbo itsva yedenda, huwandu hwevanhu hunowanzo batwa, asi chiitiko chacho chinonyanya kutaurwa nevanhu vakuru, munzvimbo yedenda, chiitiko chacho chinonyanya kuvana.

Principle

Dengue NS1 Ag-IgG/IgM Combo Test is a qualitative membrane strip based immunoassay yekuona masoja ekudzivirira chirwere chedengue (IgG neIgM) uye utachiona hwedengue NS1 antigen muSerum kana Plasma.

YeIgG/IgM Muedzo: Chishandiso chekuyedza chine: 1) burgundy colored conjugate pad ine dengue recombinant envelope antigens yakasanganiswa neColloid goridhe (dengue conjugates), 2) nitrocellulose membrane strip ine mitsara miviri yebvunzo (T1 uye T2 mitsara) uye mutsara wekutonga (C mutsara).Mutsetse weT1 wakagara wakavharwa nemasoja ekudzivirira chirwere chekudzivirira dengue, T2 mutsara wakaiswa masoja ekudzivirira chirwere kuitira kuti IgG ionekwe inorwisa dengue.Kana vhoriyamu yakakwana yebvunzo yemuenzaniso yaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.IgG anti-dengue, kana iripo mumuenzaniso, inosunga kune dengue conjugates.Iyo immunocomplex inozobatwa neiyo reagent pre-yakavharwa paT2 bhendi, ichigadzira burgundy yemavara T2 mutsara, ichiratidza dengue IgG yakanaka bvunzo mhedzisiro uye ichiratidza hutachiona huchangobva kuitika kana kudzokorora.IgM anti-dengue kana iripo mumuenzaniso inosunga kune dengue conjugates.Iyo immunocomplex inozobatwa neiyo reagent yakavharwa pamutsetse weT1, ichigadzira burgundy ine ruvara rweT1 mutsara, ichiratidza dengue IgM yakanaka bvunzo mhedzisiro uye ichiratidza hutachiona hutsva.Kusavapo kwechero T mitsara (T1 uye T2) inoratidza mhedzisiro yakaipa.

Ye NS1 Muedzo: Muiyi maitiro ekuyedza, anti-Dengue NS1 masoja ekudzivirira chirwere anovharika munharaunda yebvunzo yekaseti.Mushure meRopa Rakazara / Serum / Plasma sampuli yakaiswa mumuenzaniso mutsime, inopindirana neAnti-Dengue NS1 antibody yakavharwa masisitimu ayo akaiswa kune iyo specimen pad.Musanganiswa uyu unotama chromatographically pahurefu hwetest strip uye unopindirana neimmobilized anti-Dengue NS1 antibody.Kana mufananidzo wacho uine hutachiona hwedengue NS1 antigen, mutsara weruvara uchaonekwa munharaunda yebvunzo inoratidza mhedzisiro.Kana mufananidzo usina hutachiona hwedengue NS1 antigen, mutsara weruvara hauzoonekwa munzvimbo ino unoratidza mhedzisiro yakaipa.

Kuti ishande senzira yekudzora, mutsara weruvara unogara uchionekwa munzvimbo yekutonga inoratidza kuti vhoriyamu yakakodzera yemuenzaniso yakawedzerwa uye membrane wicking yakaitika.

Kuchengetedza uye Kugadzikana

Chengetedza seyakaputirwa muhomwe yakavharwa patembiricha yekamuri kana mufiriji (4-30 ℃ kana 40-86 ℉).Mudziyo wekuyedza wakatsiga kusvika pazuva rekupera rakadhindwa pahomwe yakavharwa.

Muedzo unofanira kuramba uri muhomwe yakavharwa kusvikira washandiswa.

Muenzaniso Kuunganidza uye Kugadzirira

1. Dengue NS1 Ag-IgG/IgM Combo Test inogona kuitwa inoshandiswa paSerum kana Plasma.

2. Kutora ropa rakazara, serum kana plasma zvienzaniso zvichitevera maitiro enguva dzose ekiriniki murabhoritari.

3. Kuongororwa kunofanira kuitwa pakarepo mushure mekuunganidza kwemuenzaniso.Usasiye zvienzaniso pane tembiricha yekamuri kwenguva yakareba.Nekuchengetedza kwenguva refu, zvienzaniso zvinofanirwa kuchengetwa pazasi-20 ℃.

4. Uya nemuenzaniso kune tembiricha yekamuri usati waedzwa.Mienzaniso yakaomeswa inofanira kunyungudutswa uye yakasanganiswa zvakanaka isati yaedzwa.Mienzaniso haifaniri kuomeswa nechando uye kunyungudutswa kakawanda.

Test Procedure

Bvumira bvunzo, sampuli, buffer uye/kana zvinodzora kusvika mukamuri tembiricha 15-30 ℃ (59-86 ℉) isati yaedzwa.

1. Hunza homwe patembiricha yekamuri usati waivhura.Bvisa mudziyo wekuyedza kubva muhomwe yakavharwa woishandisa nekukasika.Isa mudziyo wekuyedza panzvimbo yakachena uye ine mwero.

2. YeIgG/IgM Muedzo :Bata chinodonhedza pasi uye wotamisa donhwe 1 remuenzaniso (inenge 10μl) kumuenzaniso wematsime (S) echigadzirwa chekuedza, wobva wawedzera madonhwe maviri ebhafa (inenge 70μl) uye tanga timer.Ona mufananidzo uri pasi apa.

3. Ye NS1 Muedzo: Bata chinodonhedza pasi uye wotamisa 8~10 madonhwe e serum kana plasma (inenge 100μl) kune specimen tsime(S) yebvunzo mudziyo, wotanga timer.Ona mufananidzo uri pasi apa.

4. Mirira kuti mutsara wemavara (ma) uoneke.Verenga zvawanikwa pamaminitsi gumi nemashanu.Usadudzire mhedzisiro mushure memaminitsi makumi maviri.

Dudziro yeZvabuda

Zvakanaka:

YeIgG/IgM Muedzo: Kudzora mutsara uye kanenge mutsara webvunzo unoonekwa pane membrane.Kuonekwa kweT2 test line kunoratidza kuvepo kwedengue chaiyo IgG antibodies.Kuonekwa kweT1 test line kunoratidza kuvepo kwedengue chaiyo IgM masoja ekudzivirira chirwere.Uye kana zvese T1 neT2 mutsara ukaonekwa, zvinoratidza kuti kuvepo kweese ari maviri edengue chaiwo IgG neIgM masoja ekudzivirira chirwere.Iyo yakadzikira iyo antibody concentration iri, iyo ine simba mutsara wemhedzisiro.

Ye NS1 Muedzo: Mitsetse miviri inooneka.Mutsetse mumwe unofanirwa kugara uchionekwa munharaunda yekudzora mutsara (C), uye imwe mutsara unoonekera weruvara unofanirwa kuoneka mudunhu rebvunzo.

Negative:

Mutsara mumwe weruvara unoonekwa munzvimbo yekudzora (C) Hapana mutsara unooneka une ruvara unoonekwa munharaunda yebvunzo.

Haisiyo: Mutsetse wekudzora watadza kuoneka.Kusakwana specimen vhoriyamu kana zvisirizvo maitiro emaitiro ndizvo zvinonyanya zvikonzero zvekutadza kwekudzora mutsara.Wongorora maitiro uye dzokorora bvunzo nemudziyo mutsva wekuyedza.Kana dambudziko rikaramba riripo, rega kushandisa kit yekuyedza nekukasika uye ubate mushambadzi wenzvimbo yako.