Dengue Ns1 Rapid Test Cassette
Dengue Ns1 Test Device (Whole BloodSerumPlasma)
[WACHASHANDISWA]
Iyo Dengue NS1 Antigen Rapid Test Cassette/Strip is a lateral flow chromatographic immunoassay yemhando yekuona maantigen kuhutachiona hweDengue mumunhu Wese Ropa/Serum/Plasma.Inopa rubatsiro mukuongororwa kwehutachiona nehutachiona hweDengue.
[SUMMARY]
Dengue fever chirwere chechirwere chinotapukira chinokonzerwa nehutachiona hwedengue hunoparadzirwa nehumhutu.Utachiona hwehutachiona hweDengue hunogona kutungamirira kuhutachiona hunowedzera, dengue fever, dengue hemorrhagic fever, dengue hemorrhagic fever.Makiriniki ekuratidzwa kwefivha yedengue anosanganisira kuerekana kwatanga, kupisa kwakanyanya, kutemwa nemusoro, tsandanyama zvakanyanya, kurwadziwa kwebhonzo nemajoini, kuputika kweganda, kubuda ropa, kuwedzera lymph node, kuderera kwemasero machena eropa, thrombocytopenia uye zvichingodaro mune vamwe varwere.Chirwere ichi chinonyanya kufarirwa munzvimbo dzinopisa uye dzinopisa, nekuti chirwere ichi chinoparadzirwa neAides mosquito, chikonzero chakakurumbira kune imwe nguva mwaka, kuve mugore rega rega kazhinji muna Chivabvu-Mbudzi, peak iri muna Chikunguru ~ Gunyana.Munzvimbo itsva yedenda, huwandu hwevanhu hunowanzo batwa, asi chiitiko chacho chinonyanya kutaurwa nevanhu vakuru, munzvimbo yedenda, chiitiko chacho chinonyanya kuvana.
[PRINCIPLE]
Iyo Dengue NS1 Antigen Rapid Test Cassette/Strip ndeye immunoassay yakavakirwa pamusimboti weaviri antibody-sandwich maitiro.Panguva yekuyedzwa, anti-Dengue antibody inovharirwa munharaunda yebvunzo yedunhu remudziyo.Mushure mekunge Ropa Rakazara / Serum / Plasma sampuli yakaiswa mumuenzaniso mutsime, inopindirana neAnti-Dengue antibody yakavharwa zvimedu zvakaiswa kumuenzaniso pad.Musanganiswa uyu unotama chromatographically pahurefu hwetest strip uye uchipindirana neimmobilized anti-Dengue antibody.Kana mufananidzo wacho uine dengue virus antigen, mutsetse weruvara uchaonekwa mudunhu rebvunzo unoratidza mhedzisiro.Kana mufananidzo wacho usina dengue virus antigen, mutsara une ruvara hauzooneki munharaunda ino unoratidza mhedzisiro yakaipa.Kuti ishande senzira yekudzora, mutsara weruvara unogara uchionekwa munzvimbo yekutonga inoratidza kuti vhoriyamu yakakodzera yemuenzaniso yakawedzerwa uye membrane wicking yakaitika.
[KUCHENGA UYE KUTIRIRA]
Chengetedza seyakaputirwa muhomwe yakavharwa patembiricha (4-30 ℃ kana 40-86 ℉).Iyo kit yakagadzikana mukati menguva yekupera yakadhindwa pane iyo yakanyorwa.
Kana wangovhura homwe, bvunzo dzinofanirwa kushandiswa mukati meawa imwe.Kugara kwenguva refu kunzvimbo inopisa uye ine mwando kunokonzera kukanganisa kwechigadzirwa.
Iyo LOT uye zuva rekupera kwayo zvakadhindwa pane iyo yakanyorwa.
[ZVIRI MUKATI]
Muedzo unogona kushandiswa kuyedza Yese Ropa/Serum/Plasma samples.
Unganidza sampuli yeropa (ine EDTA, citrate kana heparin) nekubaya tsinga ichitevera maitiro akajairwa murabhoritari.
Kuparadzanisa serum kana plasma kubva muropa nekukurumidza sezvinobvira kudzivirira hemolytic.Shandisa zvienzaniso zvakajeka zvisina kuputswa chete.
Chengetedza sampuli pa2-8 ℃ (36-46 ℉) kana isina kuongororwa nekukurumidza.Chengetedza sampuli pa2-8 ℃ kusvika kumazuva manomwe.Zviyero zvinofanirwa kuomeswa nechando pa -20 ℃ (-4 ℉) kuitira kuchengetedza kwenguva refu.Usaomesa sampuli dzeropa rose.
Dzivisa akawanda kutonhora-kunyorova kutenderera.Usati waedza, hunza zvimedu zvakaomeswa nechando kune tembiricha yekamuri zvishoma nezvishoma uye sanganisa zvinyoro.Mienzaniso ine zvinhu zvinooneka zvinofanira kujekeswa ne centrifugation isati yaedzwa.
Usashandise sampuli dzinoratidza gross lineman, gross hemolytic kana turbidity kuitira kudzivirira kukanganiswa pakududzira kwemhedzisiro.
[TEST PROCEDURE]
- Bvumira mudziyo wekuyedza uye zvienzaniso kuti zvienzane netembiricha (15-30 ℃ kana 59-86 ℉) isati yaedzwa.
- [YeMutsetse]
1. Bvisa test strip kubva muhomwe yakavharwa uye ishandise nekukurumidza sezvinobvira.
2. Isa chidimbu chekuedza panzvimbo yakachena uye yakaenzana.
3. Kune serum kana plasma specimen: Bata dropper vertically uye uendese madonhwe matatu e serum kana plasma (inenge 100μl) kumuenzaniso wepedhi yetest strip, wozotanga timer.Ona mufananidzo uri pasi apa.
4. yezvienzaniso zveropa rose: Bata chinodonhedza pasi uye wotamisa donhwe 1 reropa rose (inenge 35μl) kumuenzaniso wepedhi yetest strip, wobva wawedzera madonhwe maviri ebuffer (inenge 70μl) uye tanga timer.Ona mufananidzo uri pasi apa.
5. Mirira kuti mutsara wemavara (ma) uoneke.Verenga zvawanikwa pamaminitsi gumi nemashanu.Usadudzire mhedzisiro mushure memaminitsi makumi maviri.
1.Bvisa kaseti yebvunzo kubva muhomwe yakavharwa uye shandisa nekukurumidza sezvinobvira.
2.Isa test cassette panzvimbo yakachena uye yepamusoro.
3.For serum kana plasma specimen: Bata dropper vertically uye uendese madonhwe matatu e serum kana plasma (inenge 100μl) kune muenzaniso wakanaka (S) wekaseti yekuedza, wozotanga timer.Ona mufananidzo uri pasi apa.
4.Nokuda kwezvienzaniso zveropa rose: Bata dropper vertically uye tumira 1 donho reropa rose (inenge 35μl) kune muenzaniso wetsime (S) yekaseti yekuongorora, wobva wawedzera madonhwe maviri e-buffer (inenge 70μl) uye tanga timer.Ona mufananidzo uri pasi apa.
5.Mirira kuti mutsara wemavara (s) uoneke.Verenga zvawanikwa pamaminitsi gumi nemashanu.Usadudzire mhedzisiro mushure memaminitsi makumi maviri.
[DUDZIRIRO YEMIZVO]
Positive:*Mitsetse miviri inooneka.Mutsara mumwe weruvara unofanirwa kunge uri munharaunda yekudzora (C), uye imwe mutsara unoonekera weruvara uri padyo unofanirwa kunge uri munharaunda yekuyedza (T).Iyi mhedzisiro yakanaka inoratidza kuvepo kwemaantigen kuDengue.
Negative: Mutsara mumwe wemavara unoonekwa munharaunda yekutonga (C).Hapana mutsara unoonekwa munzvimbo yekuyedza (T).Mhedzisiro iyi yakaipa inoratidza kusavapo kwemaantigen kuDengue.
Haisiyo: Mutsetse wekudzora watadza kuoneka.Kusakwana specimen vhoriyamu kana zvisirizvo maitiro emaitiro ndizvo zvinonyanya zvikonzero zvekutadza kwekudzora mutsara.Ongorora maitiro uye dzokorora bvunzo uchishandisa bvunzo kaseti/strip nyowani.Kana dambudziko rikaramba riripo, rega kushandisa mijenya nekukasira uye bata muparidzi wenzvimbo yako.
