[WACHASHANDISWA]

Dengue IgG/IgM Rapid Test Cassette is a lateral flow chromatogram-raphic immunoassay yemhando yekuona masoja ekudzivirira chirwere (IgG neIgM) kuhutachiona hwedengue muRopa Rese remunhu/Serum/Plasma.Inopa rubatsiro mukuongororwa kwehutachiona nehutachiona hweDengue.

[SUMMARY]

Dengue fever chirwere chinotapukira chinokonzerwa nehutachiona hwedengue hunoparadzirwa nehumhutu.Utachiona hwehutachiona hweDengue hunogona kutungamirira kuhutachiona hunowedzera, dengue fever, dengue hemorrhagic fever, dengue hemorrhagic fever.Makiriniki ekuratidzwa kwefivha yedengue anosanganisira kuerekana kwatanga, kupisa kwakanyanya, kutemwa nemusoro, tsandanyama zvakanyanya, kurwadziwa kwebhonzo nemajoini, kuputika kweganda, kubuda ropa, kuwedzera lymph node, kuderera kwemasero machena eropa, thrombocytopenia uye zvichingodaro mune vamwe varwere.Chirwere ichi chinonyanya kufarirwa munzvimbo dzinopisa uye dzinopisa, nekuti chirwere ichi chinotapuriranwa neAides mosquito, chikonzero chekuzivikanwa kune imwe nguva mwaka, kuve mugore rega rega kazhinji muna Chivabvu-Mbudzi, peak iri muna Chikunguru ~ Gunyana.Munzvimbo itsva yedenda, huwandu hwevanhu hunowanzo batwa, asi chiitiko chacho chinonyanya kutaurwa nevanhu vakuru, munzvimbo yedenda, chiitiko chacho chinowanzova vana.

[PRINCIPLE]

Dengue IgG/IgM Rapid Test Cassette imhando yemembrane strip based immunoassay yekuonekwa kwemasoja ekudzivirira chirwere chedengue (IgG neIgM) muRopa Rese remunhu/Serum/Plasma.Iyo bvunzo kaseti ine: 1) burgundy yeruvara conjugate pad ine dengue recombinant envelope antigens yakasanganiswa neColloid goridhe (dengue conjugates), 2) nitrocellulose membrane strip ine mitsara miviri yebvunzo (IgG uye IgM mitsara) uye tambo yekudzora (C mutsara. )Mutsetse weIgM wakafanoputirwa neMouse anti-Human IgM antibody, IgG mutsetse wakavharwa neMouse anti-Human IgG antibody.Kana vhoriyamu yakakwana yebvunzo yemuenzaniso yaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.IgM anti-dengue kana iripo mumuenzaniso inosunga kune dengue conjugates.Iyo immunocomplex inozobatwa neiyo reagent yakavharwa paIgM mutsara, ichigadzira burgundy yemavara IgM mutsara, ichiratidza dengue IgM yakanaka bvunzo mhedzisiro uye ichiratidza hutachiona hutsva.IgG anti-dengue, kana iripo mumuenzaniso, inosunga kune dengue conjugates.Iyo immunocomplex inozobatwa neiyo reagent pre-yakavharwa paIgG bhendi, ichigadzira burgundy yemavara IgG mutsara, ichiratidza dengue IgG yakanaka bvunzo mhedzisiro uye ichiratidza hutachiona huchangobva kuitika kana kudzokorora.Kusavapo kwechero T mitsara (IgG neIgM) inoratidza mhedzisiro yakaipa.Kuti ishande senzira yekudzora, mutsara weruvara unogara uchionekwa munzvimbo yekutonga inoratidza kuti vhoriyamu yakakodzera yemuenzaniso yakawedzerwa uye membrane wicking yakaitika.

[KUCHENGA UYE KUTIRIRA]

Chengetedza seyakaputirwa muhomwe yakavharwa patembiricha (4-30 ℃ kana 40-86 ℉).Iyo kit yakagadzikana mukati menguva yekupera yakadhindwa pane iyo yakanyorwa.

Kana wangovhura homwe, bvunzo dzinofanirwa kushandiswa mukati meawa imwe.Kugara kwenguva refu kunzvimbo inopisa uye ine mwando kunokonzera kukanganisa kwechigadzirwa.

Iyo LOT uye zuva rekupera kwayo zvakadhindwa pane iyo yakanyorwa.

[ZVIRI MUKATI]

Muedzo unogona kushandiswa kuyedza Yese Ropa/Serum/Plasma samples.

Kutora ropa rakazara, serum kana plasma zvienzaniso zvichitevera maitiro enguva dzose ekiriniki murabhoritari.

Kuparadzanisa serum kana plasma kubva muropa nekukurumidza sezvinobvira kudzivirira hemolytic.Shandisa zvienzaniso zvakajeka zvisina kuputswa chete.

Chengetedza zvienzaniso pa2-8℃ (36-46℉) kana zvisina kuongororwa nekukasira.Chengetedza sampuli pa2-8 ℃ kusvika kumazuva manomwe.Mienzaniso inofanira kuiswa muchando

-20 ℃ (-4 ℉) yekuchengetedza kwenguva refu.Usaomesa sampuli dzeropa rose.

Dzivisa akawanda kutonhora-kunyorova kutenderera.Usati waedza, hunza zvimedu zvakaomeswa nechando kune tembiricha yekamuri zvishoma nezvishoma uye sanganisa zvinyoro.Mienzaniso ine zvinhu zvinooneka zvinofanira kujekeswa ne centrifugation isati yaedzwa.

Usashandise sampuli dzinoratidza gross lineman, gross hemolytic kana turbidity kuitira kudzivirira kukanganiswa pakududzira kwemhedzisiro.

[TEST PROCEDURE]

Bvumira mudziyo wekuyedza uye zvienzaniso kuti zvienzane netembiricha (15-30 ℃ kana 59-86 ℉) isati yaedzwa.

1. Hunza homwe patembiricha yekamuri usati waivhura.Bvisa kaseti yebvunzo muhomwe yakavharwa woishandisa nekukasika.

2. Isa bvunzo kaseti panzvimbo yakachena uye yakaenzana.

3. Bata dropper vertically uye tumira 1 donho remuenzaniso (inenge 10μl) kune muenzaniso zvakanaka (S) yekaseti yekuedza, wobva wawedzera madonhwe maviri e-buffer (inenge 70μl) uye tanga timer.Ona mufananidzo uri pasi apa.

4. Mirira kuti mutsara wemavara (ma) uoneke.Verenga zvawanikwa pamaminitsi gumi nemashanu.Usadudzire mhinduro mushure memaminitsi makumi maviri.