COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal gold)
Iyo COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Goridhe) inokurumidza chromatographic immunoassay yemhando yekuonekwa kwekusarerekera masoja ekudzivirira chirwere kuCCIDID-19 muropa remunhu, serum, kana plasma serubatsiro mukuonekwa kwekuvapo kwemasoja ekudzivirira. kuCOVID-19.
Iyo novel coronaviruses ndeye β genus.COVID-19 chirwere chekufema chinotapukira.Vanhu vanowanzo batwa nedambudziko iri.Parizvino, varwere vakatapukirwa neinoveli coronavirus ndiyo honzeri yehutachiona;asymptomatic vanhu vane hutachiona vanogona zvakare kuve nehutachiona sosi.Zvichienderana neazvino epidemiological kuferefeta, iyo incubation nguva ndeye 1 kusvika 14 mazuva, kazhinji 3 kusvika 7 mazuva.Zviratidzo zvikuru zvinosanganisira fivha, kuneta uye chikosoro chakaoma.Nasal congestion, runny mhino, kurwadziwa kwehuro, myalgia uye manyoka zvinowanikwa mune zvishoma.
Iyo COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Goridhe) muyedzo unokurumidza unoshandisa musanganiswa weS-RBD antigen yakavharwa zvimedu zveruvara kuti zvionekwe kwekudzivirira masoja ekudzivirira chirwere kuCOVID-19 muropa remunhu, serum, kana plasma.
Iyo COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Goridhe) imhando yemembrane yakavakirwa immunoassay yekuonekwa kwekusarerekera masoja ekudzivirira chirwere kuCOVID-19 muropa rose, serum kana plasma.Iyo membrane yakafanoputirwa neAngiotensin I Inoshandura Enzyme 2 (ACE2) padanho rekuyedza remutsetse.Panguva yekuyedzwa, ropa rese, serum kana plasma specimen inopindirana neS-RBD yakasanganiswa colloid goridhe.Musanganiswa unosimuka uchienda kumusoro pane membrane chromatographically necapillary chiito kuita neACE2 pane membrane uye kugadzira mutsara weruvara.Kuvapo kwemutsara uyu wemavara kunoratidza mhedzisiro yakaipa, nepo kusavapo kwayo kunoratidza mhedzisiro yakanaka.Kuti ushande sekutonga kwemaitiro, mutsara wemavara unogara uchichinja kubva kuBlue kuenda kuRed munzvimbo yekudzora mutsara, zvichiratidza kuti vhoriyamu yakakodzera yemuenzaniso yakawedzerwa uye membrane wicking yakaitika.
| Yega yakarongedzerwa bvunzo zvishandiso | Chishandiso chega chega chine tambo ine mavara conjugates uye reactive reagents pre-yakapararira kumatunhu anoenderana. |
| Mapipipi anoraswa | Kuwedzera sampuli shandisa |
| Buffer | Phosphate yakavharwa nesaline uye inochengetedza |
| Pakeji isa | Kurairirwa kushanda |
Zvinhu Zvakagoverwa
| ●Edza zvishandiso | ●Droppers |
| ● Buffer | ● Package isa |
Zvinhu Zvinodiwa Asi Zvisina Kugoverwa
| ●Midziyo yekuunganidza | ●Timer |
| ●Centrifuge |
1. Kune nyanzvi in vitro diagnostic kushandiswa chete.
2. Usashandise mushure mekupera kwezuva rakaratidzwa papepa.Usashandise bvunzo kana foil pouch yakuvadzwa.Usashandise zvakare bvunzo.
3. Mushonga wekubvisa reagent une munyu wemunyu kana mushonga wacho ukasangana neganda kana ziso, geza nemvura yakawanda.
4. Dzivisa kusvibiswa-kusvibiswa kwemuenzaniso uchishandisa mudziyo mutsva wekuunganidza wemuenzaniso wega wega wakawanikwa.
5. Verenga nzira yose zvakanyatsonaka usati waedzwa.
6. Usadya, kunwa kana kuputa munzvimbo iyo mienzaniso uye kits inobatwa.Bata zvienzaniso zvese sekunge zvine zvirwere zvinotapukira.Chengetedza chenjedzo dzakamisikidzwa panjodzi dzehutachiona hwehutachiona mukati memaitiro ese uye uteedzere maitiro akajairwa ekurasa kwakaringana zvienzaniso.Pfekai zvipfeko zvinodzivirira zvakaita semabhachi emurabhoritari, magirovhosi anoraswa uye anodzivirira maziso kana zvienzaniso zvaongororwa.
7. Kana kutapukirwa nekoronavirus itsva kuchifungidzirwa zvichibva pazvinokurudzirwa nevakuru vehutano hweveruzhinji, zvienzaniso zvinofanirwa kuunganidzwa nenzira dzakakodzera dzekudzivirira kutapukira kwekoronavirus itsva uye kutumirwa kudhipatimendi rezvehutano redunhu kana remunharaunda kunoongororwa.Viral tsika haifanirwe kuedzwa mune idzi kesi kunze kwekunge BSL 3+ iripo yekugamuchira uye tsika sampuli.
8. Usachinjane kana kusanganisa reagents kubva kune akasiyana mijenya.
9. Humidity uye tembiricha zvinogona kukanganisa mhedzisiro.
10. Zvishandiso zvekuongorora zvakashandiswa zvinofanira kuraswa maererano nemitemo yemunharaunda.
1. Kiti inofanira kuchengetwa pa 2-30 ° C kusvikira zuva rekupera rakadhindwa pahomwe yakavharwa.
2. Muedzo unofanira kuramba uri muhomwe yakavharwa kusvikira washandiswa.
3. Usaomesa nechando.
4. Kuchengetedza kunofanira kutorwa kuchengetedza zvikamu zvekiti kubva pakusvibiswa.Usashandise kana paine humbowo hwekusvibiswa kwehutachiona kana kunaya.Biological kusvibiswa kwemidziyo yekuparadzira, midziyo kana reagents kunogona kutungamirira kune mhedzisiro yenhema.
Funga nezve chero zvigadzirwa zvekwakabva vanhu sehutachiona uye zvibate uchishandisa akajairwa biosafety maitiro.
Capillary Yese Ropa
Geza ruoko rwemurwere wobvumira kuti riome.Sveta ruoko usingabati chibodzwa.Bhambura ganda ne sterile lancet.Pukuta chiratidzo chekutanga cheropa.Zora ruoko zvinyoro nyoro kubva paruoko kusvika paruoko kusvika pamunwe kuita donho reropa rakatenderedzwa panzvimbo yekuboora.Wedzera mufananidzo weFingerstick Wese Ropa pachishandiswa chekuyedza uchishandisa capillary chubhu kana madonhwe ekurembera.
venous Whole Blood:
Unganidza mufananidzo weropa mulavender, yebhuruu kana yegirini yepamusoro yekuunganidza chubhu (ine EDTA, citrate kana heparin, zvakateerana muVacutainer®) neveinpuncture.
Plasma
Unganidza mufananidzo weropa mulavender, yebhuruu kana yegirini yepamusoro yekuunganidza chubhu (ine EDTA, citrate kana heparin, zvakateerana muVacutainer®) neveinpuncture.Kuparadzanisa plasma ne centrifugation.Nyatsobvisa plasma muchubhu itsva yakanyorwa kare.
Serum
Unganidza mufananidzo weropa muchubhu tsvuku yepamusoro yekuunganidza (isina anticoagulants muVacutainer®) neveinpuncture.Bvumira ropa kugwamba.Kuparadzanisa serum ne centrifugation.Nyatsobvisa serum muchubhu itsva yakanyorwa kare.
Edzai zvienzaniso nekukurumidza mushure mekuunganidza.Chengetedza sampuli pa2°C-8°C kana isina kuongororwa nekukasika.
Chengetedza sampuli pa2°C-8°C kusvika kumazuva mashanu.Mienzaniso inofanira kuiswa muchando pa -20 ° C kuitira kuchengetedza kwenguva refu.
Dzivisa akawanda kutonhora-kunyorova kutenderera.Usati waedza, hunza zvimedu zvakaomeswa nechando kune tembiricha yekamuri zvishoma nezvishoma uye sanganisa zvinyoro.Mienzaniso ine zvinhu zvinooneka zvinofanira kujekeswa ne centrifugation isati yaedzwa.Usashandise sampuli dzinoratidza gross lipemia, gross hemolysis kana turbidity kuitira kudzivirira kukanganisa pakududzira kwemhedzisiro.
Huya nemuenzaniso uye zvinhu zvekuyedza kune tembiricha yemumba Sanganisa mufananidzo zvakanaka usati waedza kana wanyungudutswa.Isa mudziyo wekuyedza panzvimbo yakachena, yakati sandara.
Kune capillary rose sampuli yeropa:
Kushandisa capillary chubhu: Zadza capillary chubhu uyekutamisa inenge 50µL (kana kuti madonhwe maviri) echigunwe cheropa rosesampuli kune specimen tsime (S) yebvunzo mudziyo, wobva wawedzera1 donho (anenge 30 μL)yeSample Diluentpakarepo mumuenzaniso mutsime.
Pamuenzaniso weropa rose:
Zadza dropper nemuenzaniso ipapokutamisa madonhwe maviri (anenge 50 μL)yemuenzaniso mutsime remuenzaniso.Kuita shuwa kuti hapana mabhuru emhepo.Zvadarokutamisa 1 donho (anenge 30 μL)yeSample Diluent nekukasira mumuenzaniso mutsime.
YePlasma / Serum sampuli:
Zadza dropper nemuenzaniso ipapokutamisa 1 donho (anenge 25 μL)yemuenzaniso mutsime remuenzaniso.Kuita shuwa kuti hapana mabhuru emhepo.Zvadarokutamisa 1 donho (anenge 30 μL) yeSample Diluent nekukasira mumuenzaniso mutsime.
Gadzirisa timer.Verenga mhinduro pamaminetsi gumi nemashanu.Usaverenge mhinduro mushure20 maminitsi.Kuti udzivise kuvhiringidzika, rasa mudziyo wekuyedza mushure mekududzira mhinduro
| ZVINHU ZVAKANAKA:
| Bhandi rimwe chete remavara rinoonekwa munharaunda yekudzora (C).Hapana bhendi reruvara rinooneka munharaunda yekuyedza (T). |
| NEGATIVE RESULT:
| Mabhandi maviri ane mavara anoonekwa pa membrane.Rimwe bhendi rinoonekwa mudunhu rekutonga (C) uye rimwe bhendi rinoonekwa mudunhu rekuyedza (T). |
| * CHERECHEDZA: Kusimba kweruvara munharaunda yebvunzo kunosiyana zvichienderana nekuwanda kwekusarerekera masoja ekudzivirira chirwere kuCOVID-19 mumuenzaniso.Naizvozvo, chero mumvuri weruvara munharaunda yebvunzo mutsara unofanirwa kunzi hauna kunaka. | |
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ZVINHU ZVISIVIRI
| Control bhendi ratadza kuoneka.Mhedzisiro kubva kune chero bvunzo iyo isina kuburitsa control band panguva yakatarwa yekuverenga inofanirwa kuregererwa.Ndokumbira utarise maitiro uye dzokorora nebvunzo nyowani.Kana dambudziko rikaramba riripo, rega kushandisa kit nekukasira uye bata muparidzi wenzvimbo yako. |
1. Internal Control:Iyi bvunzo ine yakavakirwa-mukati yekudzora chimiro, iyo C bhendi.Iyo C mutsetse inokura mushure mekuwedzera sampuli uye sampuli diluent.Zvikasadaro, ongorora maitiro ese uye dzokorora bvunzo nemudziyo mutsva.
2. Kudzora Kwekunze:Yakanaka Laboratory Kudzidzira inokurudzira kushandisa ekunze zvinodzora, zvakanaka uye zvisina kunaka (zvinopihwa pakukumbira), kuvimbisa kuita kwakaringana kweyedzo.
